Title: CLINICAL AND DIAGNOSTIC PARALLELS OF POSTACNE MANIFESTATIONS
Author: Кондакова
Last saved by: Nancy

ISSUES  OF EXTERNAL QUALITY CONTROL OF LABORATORY STUDIES OF SYPHILIS IN MEDICAL INSTITUTIONS OF UKRAINE

About the author:

V.V. Kutova, Ya.F. Kutasevich, O.N. Belokon,  T.V. Degtyar, I.N. Nikitenko, 
Yu.V. Shcherbakova

Heading | original research
Type of article | Scientific Article

Annotation

The objective of  the work is to identify systemic and random errors in the 
formulation of controlled methods and to achieve comparative results obtained 
by laboratories participating in external quality control of laboratory tests 
for syphilis.
Materials and methods. Laboratories of three levels took part in the activities 
of external quality control of serological diagnostics of syphilis: clinical 
diagnostic laboratories (CDL) of medical institutions of scientific research 
institutes of the NAMS of Ukraine, serological departments as part of the CDL 
of the dermatovenerological profile of the RDVD, CDVD.
Results.The quality of serological tests for syphilis in 21 CDL (serological 
departments) was assessed using the following methods: RMP, RPR, Rapid-test, 
TPHA, FTA-abs, ELISA in three control serum samples according to formal 
and essential characteristics. The most significant differences in the results
were revealed in the study of control serum samples with a low content of 
antibodies and those without antibodies to the causative agent of syphilis 
in  the  non-treponemal RMP test and the treponemal FTA-abs test.
Conclusions. The conducted studies have shown the high efficiency of the 
practical application of the developed system of external quality control of 
laboratory methods for diagnosing syphilis.

Tags | syphilis, external quality control, serological tests

Bibliography

1. Kutova VV, Bilokon OM,  Dehtiar TV. Zovnishnii kontrol’  yakosti serolohichnykh
doslidzhen’  na syfilis. Materialy naukovo-praktychnoi  konferentsii z mizhnarodnoiu
uchastiu «Infektsiini khvoroby suchasnosti : etiolohiia, epidemiolohiia, diahnostyka, 
likuvannia, profilaktyka, biolohichna bezpeka [External quality control of serological 
tests for syphilis. Proceedings of the scientific-practical conference with international 
participation «Infectious diseases of today: etiology, epidemiology, diagnosis, 
treatment, prevention, biologicalsafety»]. Dermatolohiya ta venerolohiya. 
2020;3(77):154–5. [in Ukr.]
2.Kutova VV, Volkoslavs’ka VM, Bіlokon’ OM. Harakteristika stanu, resursіv 
laboratorіj dermatovenerologіchnih zakladіv, yakі vikonuyut’ kompleks standartnih 
serologіchnih reakcіj na sifіlіs [Characteristics of the status, resources of laboratories 
of dermatological venereological institutions that perform a complex of standard
 serological reactions to syphilis]. Dermatologіya ta venerologіya. 2017;3(77):85–91. 
[in Ukr.]
3. Nakaz MOZ Ukraїni No286 vіd 07.06.2004 «Pro udoskonalennya dermato-
venerologіchnoї dopomogi naselennyu Ukraїni» [Order of the Ministry of Health of 
Ukraine No. 286 of June 7, 2004 «On improving dermatological and venereological 
assistance to the population of Ukraine»]. [in Ukr.]
4. Nakaz MOZ Ukraїni No997 vіd 22.11.2013. Metodichnі rekomendacії 
«Suchasnі pіdhodi do laboratornoї dіagnostiki sifіlіsu» [Order ofthe Ministry of 
Health ofUkraine No. 997 dated November 22, 2013. Methodical Recommendations 
«Modern Approaches to Laboratory Diagnosis of Syphilis»]. [in Ukr.]
5. Nakaz MOZ Ukraїni No204 vіd 29.12.1992 «Pro organіzacіyu laboratornoї 
dіagnostiki sifіlіsu v Ukraїnі» (zі zmіnami, vnesenimi zgіdno z Nakazom MOZ No
21 vіd 3.01.97) [Order of the Ministry of Health of Ukraine No. 204 dated 
December 29, 1992 «On the Organization of Laboratory Diagnosis of Syphilis
 inUkraine» (as amended in accordance with the Order of the Ministry of 
Health No. 21 dated January 3, 1997)]. [in Ukr.]
6. Kutasevich YaF, Kutovaya VV, Belokon ON, et al. Prakticheskiye aspekty 
serologicheskoy diagnostiki sifilisa na sovremennom etape [Practical
aspects of the serological diagnosis of syphilis at the presents stage]. 
Dermatologíya ta venerologíya. 2020;1(87):39–43. DOI: 10.33743/2308–
1066–2020–1–39–43 [in Russ.]
7.Kutova VV, Bilokon OM, Nikitenko IM, et al. Rol netreponemnih laboratornih 
metodіv doslіdzhennya u dіagnosticі sifіlіtichnoї іnfekcії [The role of non-repetitive
 laboratory research methods in the diagnosis of syphilitic infection]. Dermatologіya
 ta venerologіya. 2016;2(72):34–39. [in Ukr.]
8. Rotanov SV. Meropriyatiya vneshnego kontrolya kachestva serologicheskikh
Issledovaniy , napravlennykh na serodiagnostiku sifilisa[Measures of
External quality control of serological studies aimed at serodiagnosis of syphilis]. 
Vestnik dermatologii. 2008;4:9–16. [in Russ.]
9. Bondarenko GM, Unuchko SV, Nikitenko IN, et al. Sifilis: sovremennoe 
sostoyanie problemy [Syphilis: current status of the problem]. Dermatologіya 
ta venerologіya. 2018;1(79):8–12. [in Russ.]
10.Kutasevich YaF, Lіtus OІ, Kutova VV, et al. Udoskonalennya laboratornoї
 dіagnostiki sifilisu v Ukraїnі (metodichnі rekomendacії) [Improvement of laboratory 
diagnostics of syphilis in Ukraine (guidelines]. Kyiv. 2019. 28 p. [in Ukr.]
11. Janier M,  Hegyi V, Dupin N, et al. European guideline on  the management
of syphilis . J .Eur. Acad. Dermatol. Venereol. 2014;20:300–9. 
DOI: 10.1111/jdv.12734
12. Young H. Guidelines for serological testing for syphilis . Sex Transm. Infect.
 2000;76:403–5.DOI:10.1136/sti.76.5.403

Publication of the article

«DERMATOLOGY AND VENEREOLOGY» №2(92), 2021 year, 15-19 pages,
 index UDK 616.972–078.33

DOI | DOI: 10.33743/2308-1066-2021-2-15-19