DETERMINATION OF GENETIC VARIABILITY OF CLINICAL STRAINS
OF S. AUREUS EXTRACTED FROM LOCUS MORBI AND OTHER BIOTOPES IN PATIENTS WITH ATOPIC DERMATITIS
About the author:	S.K. Dzhoraeva, V.V. Goncharenko, Yu.V. Shcherbakova, 
O.V. Shchogolyeva	
Heading	ORIGINAL RESEARCHES	
Type of article	Scientific Article	
Annotation	The objective: the study of genetic affinity of S. aureus strains isolated from patients with AD
Materials and methods. Genetic variability of the 79 S. aureus isolates was evaluated according to the results of the RAPD-PCR study.
Results. It was revealed a generally high genetic polymorphism of S. aureus. It was established that with an increase in the severity of АD, the monotypism of pathogen isolates grows (p ≤ 0,05) from three different genetic clusters in patients with AD with a mild
disease course to only one cluster with a severe disease course. A high level of genetic affinity was established between S. aureus
strains isolated from different biotopes from the same patients, which for strains isolated from the nasal mucosa and from locus morbi
reached (79,5 ± 1,6)%, and for strains isolated from intact and affected skin are as increased from (75,1 ± 4,4)% to (98,8 ± 0,8)% in
parallel with the increase in the severity of the disease course in patients with AD from mild to severe forms, respectively (p ≤ 0,05).
Conclusions. Both the generally high genetic polymorphism of S. aureus strains isolated from allergodermatosеs patients and the high
affinity of isolates from different biotopes of the same patient, justify the auto-origin of the strains of staphylococci colonizing locus morbi.	
Tags	atopic dermatitis, clinical strains of S. aureus, genetic variability, RAPD-typing	
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Publication of the article	«DERMATOLOGY AND VENEREOLOGY» №4(90), 2020 year, 12-16 pages,
 index UDK 579.861.2:616.5–002–056.3(043.3)	
DOI	10.33743/2308-1066-2020-4-12-16